Monitoring dynamics of single-cell gene expression over multiple cell cycles Supplementary Information

We used two methods to characterize fluid flow through the TμC under typical run-time conditions. First, we used 0.5 μm yellow-green beads as tracers to map the flow field through the trapping region under the pressures used to deliver nutrients. We acquired a movie of bead flow at 10 frames per second and used this as input to a MATLAB implementation of the Particle Image Velocimetry (PIV) method [1]. PIV provides a vector field that accurately describes the magnitude and direction of flow, and the results affirmed our prediction of creeping flow through the trapping region (Figure S1). From this vector field, the maximum velocity through the feeder channel was found to be 75 μm/s and the mean velocity through the trapping region was 1.5 μm/s.